Fungal Cellulases I. General Properties of Unpurified Enzyme Preparations From Aspergillus Oryz.A'e
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چکیده
منابع مشابه
Aminoacylase from Aspergillus oryzae. Comparison with the pig kidney enzyme.
Aminoacylase (EC 3.5.1.14) from Aspergillus oryzae was purified from a commercially available crude material by heat treatment, precipitation by polyethyleneimine and ammoniumsulfate, gel chromatography and preparative disc-gel-electrophoresis. The purified product was homogenous as judged by polyacrylamide gel electrophoresis. SDS-gel electrophoresis, polyacrylamide-gel-gradient electrohoresis...
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Cellulose, a linear, essentially insoluble 8-1,4glucosidically linked polymer with a size of many thousand glucose units, is one of the main components of plant materials. Many micro-organisms can degrade this polymer; they produce many different cellulases which differ in both their mode of action and their overall folds. Fungal organisms which degrade cellulose produce many different cellulas...
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In a study of the mechanism of action of notatin, the flavin adenine dinucleotide-dependent glucose aerodehydrogenase of molds, Bentley and Neuberger (2) in 1949 obtained evidence suggesting that the enzyme preparation also catalyzed the mutarotation of glucose. Keilin and Hartree (3, 4) demonstrated that some notatin samples contained a second enzyme, named mutarotase, which was the most activ...
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Although the production of proteolytic enzymes by different strains of Aspergillus oryzae has been investigated in submerged culture (5, 15), in static liquid culture (3, 11), and on solid substratum (8-10), there appear to be few reports pertaining to the comparative evaluation of these different methods on the elaboration of proteinases by specific strains of A. oryzae. K. Mogi (13) compared ...
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For the construction of an overexpression system of the intracellular 1,2-alpha-mannosidase (EC 3.2.1.113) gene (msdS) from Aspergillus saitoi (now designated Aspergillus phoenicis), the N-terminal signal sequence of the gene was replaced with that of the aspergillopepsin I (EC 3.4.23.18) gene (apnS) signal, one of the same strains as described previously. Then the fused 1, 2-alpha-mannosidase ...
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1952
ISSN: 0004-9417
DOI: 10.1071/bi9520409